Temporal, population, and sexual variation in hematocrit of free-living desert tortoises: correlational tests of causal hypotheses

Abstract

Hematocrit (packed cell volume, PCV) varies seasonally in some vertebrates and often differs between the sexes and among conspecific populations. Such variation in PCV may reflect variation in nutritional health, requirements for oxygen transport (metabolic rate), or hydration. The relative importance of these influences was tested with serial measurements of PCV in two Mojave Desert populations of the desert tortoise (Gopherus agassizii) over two activity seasons, during a period with large seasonal differences in availability of food and water. Hematocrit varied widely (range = 14–40, CV = 20%), reflecting differences between populations, between sexes, between years, and over time within years. Causal hypotheses were tested by correlating PCV with physiological indicators of nutritional health (blood urea nitrogen), oxygen requirements (field metabolic rate), and hydration (length-relative mass, water-influx rate, urine osmolality, plasma sodium, and plasma osmolality). Results indicated a dominant influence of dehydration-induced hemoconcentration during this extreme drought period, although all correlations were relatively weak. Circumstantial evidence is marshalled to suggest longer term effects of nutrition (at seasonal, yearly, and population levels of variation) and perhaps oxygen requirements (driving sexual dimorphism). All these simultaneous and sometimes opposing effects interact in complex ways to produce the patterns of variation observed, and these interactions render PCV a poor indicator of physiological state in desert tortoises.