Temporal changes in intracellular free calcium levels in the developing neuroepithelium during neurulation in the chick

Abstract

Intracellular free calcium ion (Ca 2+) levels of the developing chick neuroepithelium during neural tube closure (Hamburger and Hamilton stages 3–11 of embryonic development) were determined using the Hydrophobie acetoxymethyl ester of the fluorescent dye fura-2 (fura-2/AM). Temporal changes in the free Ca 2+ level in neuroepithelial cells are correlated with the degree of folding of the neuroepithelium. The concentration of intracellular Ca 2+ in the neuroepithelium reaches its highest level when apposing neural folds are actively making contact.