Temporal and spatial variation of heavy metal concentration in four limpet species along the southeast coast of South Africa

Abstract

Rocky shores are experiencing heavy metal (HM) pollution as a result of anthropogenic activities. The information on the use of limpets Scutellastra spp. and Cellana sp. as bioindicators is limited. This study aimed to assess HM concentration in water samples, soft and shell tissues of four limpet species, Scutellastra granularis, S. longicosta; S. cochlear and Cellana capensis along the southeast coast of South Africa. Individual species were collected between 2019 and 2020 in four sites and four seasons during spring low tide. The physico-chemical parameters were simultaneously measured in situ with limpet species collection for correlation with HM. Concentration of Zn, As, Cd, Cu, Fe, Pb, Hg and Ni in limpet tissues were analysed using inductively coupled plasma optical emission spectrometry (ICP-OES). Data were explored using SPSS v26, GraphPad Prism v5, Primer v7 and MS-excel 2016. Temporal/spatial differences of physico-chemicals and HM in limpet tissues were evident. Heavy metal concentration was species specific e.g. Fe, Ni and were high in S. granularis, and Hg, As, Pb in C. capensis. The lower shore species S. longicosta and S. cochlear were notable accumulators of Zn and Cd. Limpet soft tissues concentrated 5–10 times magnitude of HM than shell tissues. This study provided a baseline information on the concentration of HM in marine limpets along the southeast coast of South Africa and suggest limpets as bioindicator species.