Abstract
We show that spectral phase shaping of fs-laser pulses can be used to optimize laser-cell membrane interactions in water environment. The energy and peak intensity thresholds required for cell poration with single pulse in the nJ range can be significantly reduced (25% reduction in energy and 88% reduction in peak intensity) by using temporal Airy pulses, controlled by positive third order dispersion, as compared to bandwidth limited pulses. Temporal Airy pulses are also effective to control the morphology of the induced pores, with prospective applications from cellular to tissue opto-surgery and transfection.
Highlights
We show that spectral phase shaping of fs-laser pulses can be used to optimize laser-cell membrane interactions in water environment
The energy and peak intensity thresholds required for cell poration with single pulse in the nJ range can be significantly reduced (25% reduction in energy and 88% reduction in peak intensity) by using temporal Airy pulses, controlled by positive third order dispersion, as compared to bandwidth limited pulses
We investigated the influence of the femtosecond laser pulse shapes on the induced surface damages on adherent cervix cancer cell line (HeLa) on glass immersed in water
Summary
We show that spectral phase shaping of fs-laser pulses can be used to optimize laser-cell membrane interactions in water environment. Germany (Received 2 February 2016; accepted 18 April 2016; published online 24 June 2016)
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