Template Stripping Method-Based Au Nanoarray for Surface-Enhanced Raman Scattering Detection of Antiepileptic Drug.

Tatsuro Endo,Kenji Yamada,Hirotaka Yamada

doi:10.3390/mi11100936

Abstract

Surface-enhanced Raman scattering (SERS) is a potential candidate for highly sensitive detection of target molecules. A SERS active substrate with a noble metal nanostructure is required for this. However, a SERS active substrate requires complicated fabrication procedures. This in turn makes it difficult to fabricate highly sensitive SERS active substrates with high reproducibility. To overcome this difficulty, a plasmonic crystal (PC) with periodic noble metal nanostructures was fabricated via the template-stripping method using a polymer-based template. Using SERS active substrates, SERS was successfully achieved using the PC by detecting low concentrations of phenobarbital which is an antiepileptic drug using a commercially available portable Raman module. The PC can be fabricated by demolding the deposited gold layer from a polymer-based template. This method is rapid, economic, and has high reproducibility. SERS can be achieved easily using this PC for a wide variety of applications such as medical, pharmaceutical, and environmental protection.

Highlights

The comprehension of biological functions like DNA replication, RNA and protein synthesis, and metabolism requires the detection and determination of target molecules in body fluids, cells, and tissues [1]
A specific color due to the localized surface plasmon resonance (LSPR) could be observed by the naked eye when the template stripping method was used
In this study, plasmonic crystal (PC) was fabricated at 200 nm of gold layer for Surface-enhanced Raman scattering (SERS) application

Summary

Introduction

The comprehension of biological functions like DNA replication, RNA and protein synthesis, and metabolism requires the detection and determination of target molecules in body fluids, cells, and tissues [1]. Target molecules can be detected and determined using liquid chromatography [2], electrophoresis [3], mass spectroscopy [4], fluorescent microscopy [5], and biochemical analytical tools, such as an enzyme-linked immunosorbent assay [6]. These instruments and methods are critical tools for understanding biological functions. Kranz et al reported the thermal shift assay to detect low molecular weight compounds [7] Using this assay, fractional binding between target molecules and recognition elements which bind to the target molecules, such as antibody and probe DNA can be detected with high sensitivity. The requirements of recognition elements such as aptamer and antibody are still unimproved

Methods

Results

Conclusion