Abstract
We demonstrated that 5-vinyldeoxyuridine (VU) and 5-carboxyvinyldeoxyuridine (CVU) can be used to photoligate a longer oligonucleotide (ODN) from smaller ODNs on a template. By performing irradiation at 366 nm, these artificial nucleotides make photoligated ODNs with high efficiency without any side reactions. Moreover, by performing irradiation at 312 nm, these photoligated ODNs were reversed to the original ODN. VU needs to be irradiated 366 nm for 6 h, but CVU needs to be irradiated at 366 nm for 15 min. Finally, we made a self-assembled structure with an ODN containing CVU and observed the photoligated ODN by photoirradiation.
Highlights
There are many methods of template-directed chemical ligation of oligonucleotides (ODNs) via a native phosphodiester bond [1] or non-native linking [2]
Four of the same ODNs were ligated by photochemical ligation using VU [13]
We demonstrated that VU, VC and CVU can be used in photochemical ligation
Summary
There are many methods of template-directed chemical ligation of oligonucleotides (ODNs) via a native phosphodiester bond [1] or non-native linking [2]. One method of template-directed chemical ligation is non-enzymatic chemical ligation [8,9]. DNA using anthracene [12] These methods have a serious problem for practical utilization, such as the low yields of photochemical ligation products and the use of short wavelengths that injure other biological components. We reported that 5-vinyldeoxyuridine (VU) can be used to photolink a longer ODN from five smaller identical ODNs on a template with high efficiency without any side reactions by photoirradiation at 366 nm [13]. This reaction ligated T and VU via [2+2].
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