Abstract
Factors which control the rate of synthesis of two distinct classes of protein, globin and non-globin proteins, by free reticulocyte ribosomes have been investigated. Three different inhibitors of protein synthesis, puromycin, cycloheximide, and hydroxylamine, were more effective in inhibiting the synthesis of globin than of non-globin protein by free reticulocyte ribosomes in the cell-free system. In contrast to the different degree of response to puromycin by free ribosomes in synthesizing globin and non-globin proteins, template activity for both classes of protein decreased at similar rates during maturation of reticulocytes in vivo and during storage of reticulocyte ribosomes at 0°. The synthesis of non-globin protein by either free or membrane-bound ribosomes was similar in that puromycin inhibited both to the same degree. The findings indicate that template activity for globin and non-globin protein in reticulocytes is not directed by a contiguous polycistronic messenger RNA, and suggest that the two classes of protein are not synthesized on the same ribosomes.
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