Temperaturwirkungen auf Isoenzymaktivitäten der Peroxidase1) und Arylesterase2) von Hordeum vulgare L.

Abstract

Barley plants were grown in controlled-environment chambers with temperatures of 14 and 22°C (exclusive 14°C in experiments on temperature effects in vitro ) with light duration of 16 h. Peroxidase and arylesterase were extracted from roots and primary leaves, respectively, and analyzed for isoenzyme patterns by disc electrophoresis. A total of 15 anodic isoperoxidases and 6 anodic isoarylesterases were tested for temperature effects on the enzyme activities in disc electropherogramms; the influence of the temperature changes 14, 22°C, 22, 14°C and 22, 14, 22°C on the activities of several isoenzymes were investigated in vivo . The kinetics of the enzyme reactions, likewise the responses of isoenzyme activities in the plants adapted to the altered temperature were distinguishable. The physiological implications of these findings are discussed with regards to disease resistance of plants.