Abstract
1. 1. Water content and distribution were examined at three epaxial muscle sites and in cardiac muscle, gut, spleen, liver and brain of summer and fall-winter populations of rainbow trout acclimated to 2°, 10° and 18°C on a seasonally “neutral” photoperiod regime (12L:12D). 2. 2. Tissue water content tended to be conservative in terms of temperature, but seasonally variable. 3. 3. Tissue water distribution was more influenced by temperature. Increases in extracellular phase volume were commonly encountered at higher temperatures, and were ordinarily accompanied by reductions in cellular phase volume. 4. 4. It is suggested that adjustments in phase volume may stem from alterations in osmotically-active organic solutes rather than inorganic electrolytes.
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