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Abstract
Detecting and characterizing polymers with a nanopore sensor requires a significant interaction between the polymer and nanopore in order for the polymer to yield measurable current blockades. To design a selective nanopore sensor, quantitative measurement of the free energy profile including the contributions of both entropy and enthalpy is essential. We have developed a laser-based tool to rapidly control the solution temperature of an alpha-hemolysin nanopore sensor, resulting in nearly continuous Arrhenius plots in less than 2 min.
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