Abstract
We engineered a synthetic temperature regulation toolbox to enable protocells to sense and respond to heat, utilizing RNA thermometers. The thermo-sensitive protocells were generated by encapsulating temperature feedback transcription/translation machinery in droplets. Based on these temperature-sensing devices, the protocells can be operated with logic AND gates, differentially processing temperature stimuli into biological signals.
Highlights
We engineered a synthetic temperature regulation toolbox to enable protocells to sense and respond to heat, utilizing RNA thermometers
cell-free protein synthesis (CFPS) reconstitutes the components of the cellular transcription and translation machinery for protein synthesis de novo, avoiding purification
We describe the engineering of a protocell that can respond to temperature stimuli, using temperature sensitive non-coding RNA sequences that we refer to as RNA thermometers (RNATs)
Summary
We engineered a synthetic temperature regulation toolbox to enable protocells to sense and respond to heat, utilizing RNA thermometers. The thermo-sensitive protocells were generated by encapsulating temperature feedback transcription/translation machinery in droplets. Based on these temperature-sensing devices, the protocells can be operated with logic AND gates, differentially processing temperature stimuli into biological signals.
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