Abstract
Six temperature-sensitive (ts) mutants were isolated from the progeny of wildtype influenza A virus grown in the presence of 5-fluorouracil. All mutants had the efficiency of plating lower than 10−4 at 38° C compared with 31° C. One mutant (ts- 5) failed to produce hemagglutinin at the nonpermissive temperature. Temperature-shift experiments revealed that the temperature-sensitive step ofts-5 resides in the process normally taking place at 4–6 hours post infection. After mixed infection ofts-5 andts-9, different from each other in physiological defect, the occurrence of both complementation and recombination was demonstrated, confirming that they carry genetic defects at different genes.
Sign-in/Register to access full text options 
Free) 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a call
