Abstract
Following chemical mutagenesis of Sindbis virus, two viral clones (clone 35 and clone 58) were isolated which at 34.5° had a relative plaquing efficiency on Aedes albopictus mosquito cells 10 4- to 10 5-fold lower than that of wild type virus. Standard growth curves showed that at 34.5° the viral mutants were restricted in mosquito cells but not in primary cultures of chick embryo fibroblasts (CEF). Adsorption of intact virus particles of clone 35 and clone 58 to mosquito cells was as efficient as that observed with wild type virions. However, transfection of mosquito cells with viral RNA of these mutant clones gave a significantly lower plaquing efficiency than infectious RNA from standard virus. Both mutants were temperature sensitive in CEF as well as in A. albopictus cells. At the nonpermissive temperature they were RNA − and by complementation analysis were assigned to group F. Clones 35 and 58 did not complement each other.
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