Abstract
Tisbe holothuriae was collected from the Saronicos Gulf of Greece (38%.) and reared under laboratory conditions. The effects of temperature (14, 19, 24 °C), salinity (26, 32, 38, 44%., for each tested temperature), two modalities of light: photoperiod (LD 24:0, 18:6, 12:12, 6:18, 0:24 h), spectral composition (blue, green and red wavelengths) on mortality during larval development and sex ratio of adults were studied. Survival and proportion of females decrease as temperature declines from medium levels (19°C) and salinity from normal levels (38%.). The effects of low temperatures and salinities appear to be pronounced on both parameters studied. Multiple regression analysis proved the quadratic and linear effects of temperature to be more important factors affecting the time required for the death of 50% of the initial nauplii than those of salinity. The linear effect of salinity is a more important factor affecting the sex ratio than temperature. Continuous illumination and long-day photoperiod significantly reduce the larval survival and the proportion of females. Red and green wavelengths markedly increase the percentage mortality. Spectral composition seems not to influence sex ratio.
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