Temperature-programmed reversed-phase liquid chromatography with packed fused-silica columns

Abstract

Temperature-programmed separations using fused-silica columns with reversed-phase packings are described. The columns were specially designed for temperature programming. This allows their operation at elevated temperatures under high pressure and the use of an UV detector at room temperature without back pressure. Temperature programming has successfully been applied to the separation of p-nitrobenzyl esters of fatty acids, which are eluted over a wide range of capacity factors under isothermal conditions. It is also shown that fused-silica columns are superior to glass capillary columns, and 1-m fused-silica columns with 10- and 5-μm packings generate 60,000 and 90,000 theoretical plates, respectively.