Temperature, pH and Media Influence on Lipase Stability

Abstract

An extensive study on the stability of Chromobacterium viscosum lipase B was carried out with emphasis on the influence of pH (6–8) and temperature (30–50 °C) on lipase in aqueous and reversed micellar (AOT/isooctane) systems. Two major effects of the encapsulation of the lipase in the micelles were observed: greater stability, at pH 7 and 8, and higher resistance to thermal deactivation. The stability of the lipase was strongly affected by the pH, especially in reversed micelles. The lipase in organic media was also used in a continuous reversed micellar membrane reactor for over a week without significant loss of activity.