Abstract
To study the temperature influence on both Penicillium citrinum growth and citrinin accumulation, a strain isolated from corn was cultured on Czapek agar with maize extract at 15, 20, 25 and 30 °C. Radial growth rate and lag phase were determined from the increase in colony diameter with time. The optimal temperature for P. citrinum growth was 30°C. Citrinin extracted from the agar medium was determined by thin layer chromatography. Citrinin accumulation kinetics were analyzed by fitting the data to curves generated by using a logistic function. The parameters obtained from this equation demonstrated, for all temperatures studied, that the maximum citrinin accumulation by P. citrinum on Czapek agar with maize extract was at about 30°C. At 37°C a rapid decrease in the citrinin concentration was observed after a maximal value was reached.
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