TEMPERATURE‐INDUCED CAVITIES AND SPECIALIZED PARENCHYMA CELLS IN THE VASCULAR CYLINDER OF PEA ROOTS

Abstract

Pea seeds (Pisum sativum L.) of six cultivars were planted in the field, in the greenhouse, or in growth chambers, in five different media, in light or dark, and at various temperatures (10–32 C). Under all conditions above 15 C the central portion of the vascular cylinder, in all cultivars except “Ageotropum,” tended to form cavities in almost every primary root examined. These cavities then became filled by the ingrowth of specialized parenchyma cells (SP cells). The formation of cavities and SP cells was temperature dependent since the roots grown below 15 C always formed central metaxylem tracheary elements (MTEs), without cavities and SP cells. Cavities and SP cells did not form over the entire root length. When the roots were longer than 3 cm, they started to form cavities and SP cells and continued for an additional 10–30 cm. After that, late MTEs formed in the central vascular cylinder, and no cavities and SP cells occurred regardless of temperature. Within an individual root grown above 15 C, cavities and SP cells tended to form during periods of fast growth, while during periods of slow growth large central MTEs formed instead.