Abstract
Relative growth is often used as a phenotypic measure to distinguish mutant and wild-type yeast or bacterial strains. Differential growth as a function of temperature is a convenient and accurate means of analyzing differences between strains. Slight differences in the genotypes of two strains frequently result in differential growth of the two strains as a function of temperature. We have developed a chamber for the simultaneous growth of multiple strains in microtiter plates along a temperature gradient. Image analysis was used to determine colony area and number at various times as a function of temperature. This chamber reduces the time required and increases the accuracy in measuring growth as a function of temperature. This occurs by allowing relative growth to be measured along a temperature gradient where all other conditions are constant. Two strains of yeast (Saccharomyces cerevisiae) with a known difference in temperature dependence of growth were used to demonstrate the performance of this chamber.
Sign-in/Register to access full text options 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
