Abstract
Raman spectra of collagen and DNA were discussed at different temperatures. The temperature-dependence of Raman intensity was obtained in the region from −150 to 200 °C. Four denaturation points at 0, 40, 68 and 90 °C of collagen and two peaks at 38 and 82 °C for DNA were obtained. The wavenumbers of many vibrational modes were found to increase for lower temperature, but the peak at 1302 cm −1 of collagen and the peak at 1101 cm −1 of DNA showed the opposite trend. In all of the vibrational modes of DNA, the bases showed the most sensitive to different temperatures and there is a pronounced shift of bands at 70 °C, the starting point of denaturation.
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