Temperature dependence of the crossbridge cycle during unloaded shortening and maximum isometric tetanus in frog skeletal muscle.

Abstract

The primary objective of this study was to determine if the rate limiting step in the crossbridge cycle was the same during maximum rate of shortening and during maintenance of maximum tension in an isometric contraction. To this end the temperature dependence, Q10, of the crossbridge cycle was estimated during unloaded shortening and maximum isometric tetanus. Isolated semitendinosus muscles from the frog were studied at 0 and 10 degrees C. Crossbridge cycling during unloaded shortening was determined from the velocity of unloaded shortening estimated by the slack step technique. Crossbridge cycling during maintained isometric tetanus was determined from the steady rate of energy liberation during the tetanus after allowance for energy liberation due to Ca2+ cycling. The Q10 of the velocity of unloaded shortening was 2.5 and that of the steady rate of energy liberation was 4.6. After correction for the temperature dependence of energy liberation associated with Ca2+ cycling (5.7), the estimated Q10 of the steady rate of energy liberation became 3.9. These estimates of the Q10 of the crossbridge cycle are significantly different. These results support the conclusion that the rate limiting steps during unloaded shortening and maximum isometric force maintenance occur at different steps in the crossbridge cycle. Further the high Q10 of the energy liberation due to Ca2+ cycling may relate to the high concentration of parvalbumin in frog muscle. A second objective of this study was to document in the same muscle the variation of Q10s of mechanical and energetic properties of contraction. Over this temperature range the Q10s ranged from 1.1 to 5.7.