Temperature dependence of phosphate entry into stored human erythrocytes

Abstract

The kinetics of phosphate entry into 3–4-week-old, cold stored human erythrocytes incubated in the absence of metabolic substrate at 37°C is similar to uptake by fresh cells. At temperatures below 37°C an apparent activation energy of 20 kcal/mole was measured. At temperatures above 37°C the apparent activation energy was 7 kcal/ mole. A model for phosphate entry into the stored erythrocyte is presented.