Abstract
The rate of regeneration of frog 580 cone pigment (λ max = 580 nm) is measured at 23.11 and 4°C following both flash (1 msec) and continuous (5-min) bleaches. We find that: (1) at 23°C the cone pigment regenerates more rapidly following the flash bleach ( t 1 = 1.5 min ) than following the continuous bleach ( t 1 = 8.5min ): (2) regeneration following the flash bleach is more temperature-dependent than the regeneration following the continuous bleach. This difference in temperature dependence results in almost equal rates of regeneration when the temperature is lowered to 4°C (flash bleach, t 1 = 15 min : continuous bleach. t 1 = 14 min ): (3) more pigment regenerates at lower temperatures. Possible mechanisms responsible for these results are discussed.
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