Abstract

Laboratory evaluations were conducted of the rate of ammonia biooxidation to nitrite by an autotrophic culture of nitrifiers. Ammonia oxidation biokinetics were found to follow Michaelis-Menten-type relationships. Alterations in Michaelis-Menten kinetics were quantified with elevated temperatures and selected trace organics typical of steel industry type wastewaters. Based on data obtained, the toxic inhibition to biological nitrification decreased in the order of free cyanide, coal tar acid phenolics (derived from a coke plant), phenol, 2,3,6-trimethylphenol, 2-ethylpyridine, 2,4,6-trimethylphenol, complexed cyanides and thiocyanate. All substances except free cyanide appear to follow a “shoulder effect” where low levels of inhibitor had no influence on rates of biological nitrification while higher levels had profound effects. Upper temperature limits for stable optimum nitrification is about 30°C with decreasing rates of nitrification on either side of this optimum. Nitrification rates approach zero as temperatures of wastewater approach 45°C.

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