Abstract
This paper developed a temperature control system in order to carry out a real time and continuous observation on living cells under microscope. In this system, transparent indium tin oxid (ITO) conductive film was used as a heater, temperature was measured by the temperature dependence of resistance behavior of the ITO film, C8051F340 single-chip was used as the control hardware core, the modified PID algorithm and pulse width modulation(PWM) were adopt as the key control software programs. The temperature system had a simple structure without added temperature sensor, an uniform temperature distribution within ±1 °C on the ITO film surface and temperature control precision of ±0.2 °C could be obtained. The system could maintain a good growth state for BHK-21 living cell under microscope for 48 hours.
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