Abstract
A temperature-control system was developed in order to maintain cultured rat heart cells at a constant temperature within the range of 20–40 deg C during microscopy. A linearised thermistor bridge was used in conjunction with a 3-term controller and negative feedback to achieve a temperature stability of ±0·05 deg C. Cooling fluid passing through the heating unit improved transient response and acted as an antagonist to the heating coil. The interaction between the heating and cooling elements helped to maintain better temperature regulation and allowed for operation below room temperature.
Published Version
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