Abstract
Introduction: The widely used method for transformation was the heat shock method, which was influenced by the time and temperature of heat shock treatment. However, there was a lack of research on the optimal temperature for pGEM-T plasmid transformation in Escherichia coli JM109. This study uses the heat shock method to determine the optimum temperature and time for pGEM-T transformation in Escherichia coli JM109.
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
