Abstract
Nanofibrils are nano-sized fibers. One of the materials that can be used to make nanofibrils is whey protein isolate. Nanofibrils can be formed by heating whey protein isolate at a certain temperature and for a certain duration. This study examines the effect of heating temperature and heating time on the formation of nanofibrils. WPI was formed into nanofibrils by heating the WPI solutions at 70 °C, 80 °C and 90 °C for 0, 2, 4, 6, 8, 10, 12, 14, 16, 18 and 20 hours. WPI nanofibrils in the solutions is observed by placing the solutions in between a cross-polariser. Based on the results of the study, it shows that heating at 70 °C was observed for 2 to 20 hours and did not form nanofibrils whey protein isolate. The nanofibrils were formed for heating at 80 °C for 4 hours, but the population was still small; along with the length of heating the population of nanofibrils is increasing. Heating at 90 °C for 2 hours the nanofibrils began to form; the population is increasing along with the length of heating. Based on the image, the nanofibrils heating at 80 °C is similar to 90 °C heating. Heating 80 °C is sufficient for the process of forming whey protein nanofibrils.
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