Abstract
Panellus stypticus is a white-rot basidiomycete of world-wide distribution. The 0.5 cm to 2.5 cm diameter gilled basidiocarps are evident from late summer through winter, usually on dead hardwood limbs. Basidiocarps can revive from desiccation when moistened, often generating more basidiospores. Basidiospores are thin-walled, hyaline and amyloid (i.e., they stain with Melzer’s iodine solution). In the present study the ultrastructure of events in basidiosporogenesis in P. stypticus was investigated using transmission electron microscopy (TEM). Conventional fixation (CF), using aqueous-based fixatives, and plunge freezing followed by freeze substitution (FS) fixation procedures were employed. Gold-tagged wheat germ agglutinin (WGA), Riccinis communis agglutinin (RcA) and α-amylase were used to investigate the composition of cytoplasmic and extra-cytoplasmic polysaccharides and cell walls, and to localize polysaccharides which may be involved in the ability to revive from desiccation.
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Schedule a callMore From: Proceedings, annual meeting, Electron Microscopy Society of America
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