Abstract
Somatic growth is considered to affect pace of the telomere attrition in vertebrates. As normally developed and dwarf fish differ in the body size we have decided to compare telomere length in the rainbow trout (Oncorhynchus mykiss) with normal growth and with growth reduced due to the dwarf condition. Examined 1-year-old fish with normal and dwarf appearance were siblings originated from androgenetic fully homozygous doubled haploid (DH) line of rainbow trout. Particular dwarf individuals had body deformities such as humpback, kyphosis, and lordosis. Somatic cells of examined rainbow trout had an average telomere length between 17 and 20 kb, comparable in females and males. Dwarf rainbow trout exhibited significantly lower body length and weight than their normally developed siblings even though no differences in the telomere length were found between these fishes. Statistical analysis did not exhibit any correlation between body size and the telomere length. Equal length of telomeres observed in the studied normal and dwarf rainbow trout suggests morphological and physiological differences in fish with different growth rates do not affect dynamics of telomeric DNA. Or any variation in the telomere length might have been levelled by telomerase that in rainbow trout is active in all tissues irrespective of the individual developmental stage.
Highlights
Telomeres, the nucleoprotein complexes located at the ends of linear eukaryotic chromosomes that assure their complete replication, protect their ends from DNA degradation, DNACommunicated by Maciej Szydlowski1 3 Vol.:(0123456789)Journal of Applied Genetics stem cells, and tumors (Chan and Blackburn 2004)
Dwarf rainbow trout exhibited significantly lower body length and weight than their normally developed siblings even though no differences in the telomere length were found between these fishes
Androgenetic doubled haploids (DHs) and their normal heterozygous siblings studied in this research were produced and maintained using gamete donors from the spring spawning broodstock of rainbow trout from Rutki strain reared in the Department of Salmonid Research (DSR), Inland Fisheries Institute in Olsztyn (IFI), Rutki, Poland (Polonis et al 2021)
Summary
The nucleoprotein complexes located at the ends of linear eukaryotic chromosomes that assure their complete replication, protect their ends from DNA degradation, DNACommunicated by Maciej Szydlowski1 3 Vol.:(0123456789)Journal of Applied Genetics stem cells, and tumors (Chan and Blackburn 2004). Due to the increased metabolic costs, somatic growth in individuals with spinal deformities may be retarded what usually results in dwarfism (Witten et al 2009; McMenamin et al 2013; Shao et al 2019). In experiments concerning production of rainbow trout (Oncorhynchus mykiss) androgenetic and gynogenetic doubled haploids (DHs), dwarf individuals with spinal deformities have been frequently observed (Ocalewicz et al 2010, 2013, among others). Growth retardation makes dwarf fish interesting objects to study dynamics of the telomeric DNA. Dwarf fish may have decreased ratio of cell divisions; on the other hand, such fish presumably show increased metabolic costs (Shao et al 2019) what may affect pace of the telomere shortening. The main goal of the present research was to examine changes of the telomere length in the dwarf and normal rainbow trout
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