Abstract
Anagrus nilaparvatae (Pang et Wang) is an egg-parasitoid of rice brown planthopper (Nilaparvata lugens Stål). The objective of this study was to develop a mass-rearing technique of A. nilaparvatae. Mass-rearing was done using a plastic box (14 cm × 18.5 cm × 18.5 cm, made of plastic-mica, the upper side equipped with a 10 cm test tube), placed on a tray with rice seedlings containing an estimated of 11,130 eggs of N. lugens. About 355 adult parasitoids were successfully released per box when each box was infested with 50 parasitoids. The number of parasitoids produced were reduced to 164 adults when the infestation was made at 100 parasitoids per box. The emergence of parasitoids started around 07:00 am until 04:00 pm with the highest rate (36.2%) occured at 10:00 am. One plastic box cost only Rp10.000,-; thus the estimate cost to produce one parasitoid using this tecnique was Rp60,- These findings suggest that the plastik box is a simple and inexpensive technique for mass-rearing of A. nilaparvatae.
Highlights
Pengendalian hayati dalam skala luas memerlukan jumlah agens hayati yang banyak sehingga perlu usaha pembiakan massal
The emergence of parasitoids started around 07:00 am until 04:00 pm with the highest rate (36.2%) occured at 10:00 am
One plastic box cost only Rp10.000,; thus the estimate cost to produce one parasitoid using this tecnique was Rp60, These findings suggest that the plastik box is a simple and inexpensive technique for mass-rearing of A. nilaparvatae
Summary
Lokasi dan waktu penelitian Penelitian dilaksanakan di Laboratorium Toksikologi Pestisida dan rumah kaca, Jurusan Hama dan Penyakit Tumbuhan, Fakultas Pertanian, Universitas Gadjah Mada, Yogyakarta. Tanaman padi umur satu bulan dalam pot plastik hitam (diameter atas 18 cm, diameter bawah 12 cm, tinggi 14 cm) diinfestasi dengan 10 betina N. lugens yang siap bertelur selama dua hari, selanjutnya ditempatkan pada pertanaman padi di sawah yang merupakan lokasi serangan N. lugens. Seratus imago N. lugens siap bertelur diinfestasikan pada bibit padi umur satu minggu dalam baki plastik ukuran 15 cm x 20 cm x 3 cm dan ditutup dengan kotak plastik. 2. Laju pemunculan parasitoid Laju pemunculan parasitoid diamati dengan menghitung jumlah parasitoid yang muncul setiap jam mulai dari jam 07:00 sampai 16:00 WIB dalam satu hari. Yang muncul per hari dari kotak plastik yang diinfestasi dengan 50 dan 100 imago parasitoid. Pada setiap jam panen dari kotak plastik yang diinfestasi dengan 100 gravid Nilaparvata lugens dan 50 parasitoid Anagrus sp. Biaya perbanyakan massal parasitoid Anagrus nilaparvatae per satu kotak plastik
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