Tekirdağ ilinde kirazda Bakteriyel kanser hastalığına neden olan hastalık etmenlerinin karakterizasyonu

Abstract

Sweet cherry (Prunus avium L.) is one of the most important fruit trees grown in Tekirdag. Causal disease agent(s) of bacterial canker which reduces yield and quality of sweet cherry fruit and cause death of trees were investigated. For this purpose a survey study was conducted in 2012-2013 and 150 infected plant samples were collected from symptomatic trees. As a result of survey studies, bacterial canker was determined in all orchards, while the prevalence rate of diseases was determined as 20-57% and its severity estimated as 20-85% in surveyed orchards. As a result of isolation studies, 60 bacterial isolates of Pseudomonas syringae were obtained. Results obtained from LOPAT, oxidase, pectolytic activity and arginine dihydrolase revealed that all isolates were recorded as negative for oxidase, pectolytic activity and arginine dihydrolase, but were positive on tobacco and levan production. Further GATTa characters of tested isolates were found as (+ + - -) for 28 isolates identified as Pseudomonas syringae pv. morsprunorum, while 32 isolates were recorded as (- - + +), identified as Pseudomonas syringae pv. syringae. Coincidentally 62-90% of selected isolates showed similarity according to fatty asit methyl ester analaysis. By using specific primers, 28 isolates formed 650 bp repeatable band so identified as Pseudomonas syringae pv. morsprunorum, 32 isolates formed 752 bp repeatable band so identified as Pseudomonas syringae pv. syringae.