TECHNOLOGY OF REGENERATION OF THE BONE TISSUE IN A ROTATIONAL BIOREACTOR: FLUID FLOW SIMULATION AND LASER-INDUCED FLUORESCENCE DIAGNOSTICS

Abstract

A technology of bone tissue cultivation on a thin scaffold in a rotational bioreactor is developed. An optimal regime of cell material cultivation is determined for testing the method, and an optical method for diagnostics of the bone growth dynamics during its cultivation is found. Numerical simulations of the fluid flow in the bioreactor make it possible to significantly simplify the medical experiment and to choose the optimal values of the rotation frequencies and shear stresses acting on the cell material placed on the scaffold. The optical diagnostics of the scaffold samples in the course of dynamic cultivation in the bioreactor is performed by the method of laser-induced fluorescence. An algorithm based on the principal component analysis is applied to analyze the spectral data; as a result, the spectra of excitation and fluorescent emission of the basic fluorescent substances in the sample (tyrosine and tryptophan amino acids, structural protein (collagen), and fluorescent structures of polycaprolactone) are calculated. It is found that the contribution of the component corresponding to collagen in the samples increases in the process of dynamic cultivation, which testifies to effective formation of the extracellular matrix of the bone.