Abstract
ABSTRACTPurposeAs a classical xenotransplantation model, porcine kidneys have been transplanted into the lower abdomen of non-human primates. However, we have improved upon this model by using size-matched grafting in the orthotopic position. The beneficial aspects and surgical details of our method are reported herein.MethodsDonors were two newborn pigs (weighting 5 to 6 kg) and recipients were two cynomolgus monkeys (weighting, approximately, 7 kg). After bilateral nephrectomy, kidneys were cold-transported in Euro-Collins solution. The porcine kidney was transplanted to the site of a left nephrectomy and fixed to the peritoneum.ResultsKidneys transplanted to the lower abdomen by the conventional method were more susceptible to torsion of the renal vein (two cases). In contrast, early-stage blood flow insufficiency did not occur in orthotopic transplants of theleft kidney.ConclusionsSize-matched porcine-primate renal grafting using our method of transplanting tothe natural position of the kidneys contributes to stable post-transplant blood flow to the kidney.
Highlights
Advancements in renal transplant therapy in the 20th century involved testing new immunosuppressant therapies in primate models and developing numerous primate-to-primate transplant models[1]
Previous preclinical studies have used renal transplant models with porcine donors, and monkey[3,4] and baboon[5,6,7] recipients, both of which are non-human primates and require surgery with accurate techniques, in order to minimize the number of animals used, out of respect for animal welfare
These experimental renal transplants require anastomosis of the transplanted renal artery to be made to the abdominal aorta at a lower level than the recipient’s renal vein, as well as orthotopic anastomosis of the transplanted renal vein to the inferior vena cava[1,4]
Summary
Advancements in renal transplant therapy in the 20th century involved testing new immunosuppressant therapies in primate models and developing numerous primate-to-primate transplant models[1]. Previous preclinical studies have used renal transplant models with porcine donors, and monkey[3,4] and baboon[5,6,7] recipients, both of which are non-human primates and require surgery with accurate techniques, in order to minimize the number of animals used, out of respect for animal welfare. There are no models of orthotopic transplants of porcine kidneys with blood vessels in non-human primates[4] These experimental renal transplants require anastomosis of the transplanted renal artery to be made to the abdominal aorta at a lower level than the recipient’s renal vein, as well as orthotopic anastomosis of the transplanted renal vein to the inferior vena cava[1,4]. Based on the experience accumulated far, when the recipient animal is a quadruped, the transplanted kidney hangs from the aorta or inferior vena cava, and, the blood flow is less likely to be impaired[8]
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