Techniques in Glycobiology

Abstract

Mass spectrometry and NMR: new frontiers in nuclear magnetic resonance spectroscopy - use of the Nano.NMR probe for the analysis of microgram quantities of complex carbohydrates analysis of anionic glycoconjugates by delayed extraction matrix-assisted laser desorption time-of-flight mass spectrometry. Glycolipids and the new physical methods: microscale analysis of glycosphingolipids by thin-layer chromatography blotting high-performance liquid chromatography of gangliosides a sensitive cell suspension enzyme-linked immunosorbent assay measuring the density of cell surface ganglioside and carbohydrate antigens. Protein 0-glycosylation: effects of 0-glycosylation on protein structure - the role and characterization of heavily 0-glycosylated mucin domains predicting mucin-type 0-glycosylation sites experimental approaches to studying 0-glycosylation in vivo. Labelling oligosaccharides with chromophore and fluorophores: highly sensitive pre-column derivation procedures for quantitative determination of monosaccharides, sialic acids, and amino sugar alcohols of glycoproteins by reversed phase high-performance liquid chromatography glycan labelling with the fluorophores 2-aminobenzamide and anthranilic acid. Strategies for oligosaccharides separations and sequencing: discovery and uses of novel glucosidases: resolution of oligosaccharides in capillary electrophoresis a comparison of oligosaccharides profiling methods. Plant glycobiology: recent developments in starch structure analysis using high-performance anion-exchange chromatography with pulsed amperometric detection red seaweed galactants - methodology for the structural determination of corallinan, a different agaroid. (Part contents)>