Techniques for the Clonal Culture of Hemopoietic Cells in Semisolid Medium

Abstract

Many different methods have now been developed for obtaining colony formation in semisolid agar medium by animal or human hemopoietic cells. Rather than provide an extensive description of all current methods for each separate type of hemopoietic cell, it is proposed to give a general discussion of the principles and to describe one standard method for each cell type. References to other methods will be given where these alternative methods have advantages for special purposes.