Abstract
ABSTRACT. Techniques for investigating pheromones responsible for clustering in Apis mellifera are described. Stable queenless clusters were formed in response to synthetic Nasonov pheromone mixed with (E)‐9‐oxo‐2‐decenoic acid. This mixture plus the addition of (E)‐9‐hydroxy‐2‐decenoic acid was less potent in initiating cluster formation than it was without this addition, but the clusters once formed sometimes grew larger. Other unknown components from the queen's mandibular glands encouraged cluster formation.
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