Abstract
When lymphocytes stored in the frozen state are used in the lymphocytotoxic test the fraction of cells dying after thawing must not exceed 10–20%. The effect of various concentrations of dimethyl sulfoxide (DMSO), the composition of the medium, and other parameters accordingly was investigated in order to work out optimal conditions for freezing human lymphocytes. The best results were obtained by freezing the cells in autologous serum with 12.5% DMSO. However, sufficiently good results also were obtained by the use of AB serum and 20% bovine serum in Eagle's medium.
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