Abstract
An improved technique using agar in Petri dishes has been developed for single-spore inoculation of brassicas by Plasmodiophora brassicae. The agar dish technique gave a higher percentage infection than the paraffin drop method. When single spore isolations were made from two populations, ECD 16/31/31 and ECD 22/31/31, they gave rise to a number of different physiologic races, probably indicating that the original populations were mixed. When these single spore isolates were re-tested by successive multisporous transfer through ECD hosts, they continued to show the same host specificity.
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