Abstract
A culture system designed to study root growth and mycorrhizal formation is described. This technique allows shoots of seedlings to grow in the air while roots of the seedlings are growing in an aseptic condition where their growth and differentiation, fungal colonization, mantle formation, and mycorrhizal association can be observed. Packing vermiculite and ground peat moss into the tubes stabilized the pH. This technique has been successful with two pine species and three mycorrhizal fungi.
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