Abstract
Eggs of two species of planthoppers, Nilaparvata lugens (Stål) and Sogatella furcifera (Horváth), and a leafhopper, Nephotettix virescens (Distant), were revealed in situ in susceptible and resistant rice plants using a simple staining technique. Rice plants containing insect eggs were bleached in boiling water for 5–7 min, kept in 95% ethyl alcohol for 3 days, rinsed in water, and then immersed in 1% aqueous acid fuchsin solution for 2 days. Plants were then washed under running water until stained eggs were differentiated from destained plant tissue. Eggs were counted using a 20 × binocular microscope. Results indicated that indiscriminate egg laying by N. lugens, S. furcifera, and N. virescens females rendered all test cultivars equally suitable for oviposition; however, significantly fewer eggs hatched on resistant rice cultivars than on susceptible ones.
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