Abstract
A NEW MICROSCOPE improves the three-dimensional resolution of fluorescence imaging to an unprecedented level of detail. The instrument’s resolution is better than 20 nm, which is at least twice as good as resolutions previously reported for 3-D fluorescence imaging. Harald F. Hess of Howard Hughes Medical Institute’s Janelia Farm Research Campus, Jennifer Lippincott-Schwartz of the National Institutes of Health, and coworkers use their new method, called interferometric photoactivated localization microscopy (iPALM), to resolve such structures as microtubules, cell membranes, and receptors in the endoplasmic reticulum, the cellular organelle where protein and lipid synthesis occurs. This is the first time microtubules, components of cells’ cytoskeletons that are about 25 nm in diameter, have been resolved by a method other than electron microscopy, the researchers say. Furthermore, iPALM provides molecular information that is not available from electron microscopy. The method can “dissect the molecular di...
Sign-in/Register to access full text options 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
