Abstract
A new strategy has been developed for detecting bond-breaking and bond-forming chemical reactions. Potential applications of the technique include detection of catalysis or inhibitory activity in combinatorial libraries and development of drugs and diagnostic reagents. The new technique, developed by Hicham Fenniri, Kim D. Janda, and Richard A. Lerner of the departments of molecular biology and chemistry at Scripps Research Institute, La Jolla, Calif., uses an reaction to monitor the making and breaking of chemical bonds with very high sensitivity [Proc. Natl. Acad. Sci. USA, 92, 2278 (1995)]. An encoded reaction is an assembly containing three linked elements: a glass bead, the substrate or substrates of a bond-making or bond-breaking reaction, and a DNA polynucleotide capable of being amplified by polymerase chain reaction (PCR). The term cassette refers to the versatile manner with which different substrates can be inserted (like tapes) into a reaction-cassette as...
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