Abstract
OBJECTIVE: IMSI which is based on Differential Interference Contrast (DIC) has the following drawbacks: the need for oil immersion and glass bottom dish; low working distance (WD); separation of sperm (SP) selection from SP injection on different microscope; no glass heated stage. The objectives of our study were: 1)to develop HMC microscopic setup to detect vacuoles in SP without the above listed drawbacks; 2)to compare the outcome of HMC-IMSI with conventional ICSI.
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