Abstract
ABSTRACT We analyzed methyl esters of long-chain fatty acids (LCFA) in fecal samples obtained from crossbred heifers to determine whether these compounds could be useful indicators of the presence or absence of Escherichia coli O157. Fecal samples were characterized for presence or absence of E. coli O157 using immunomagnetic separation, LCFA were determined by gas chromatography, and a stepwise regression technique was used to evaluate the relationship between presence of E. coli O157 and LCFA. Of the 39 LCFA evaluated, only eicosapentaenoic acid concentration was associated with presence of the pathogen in feces (P
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