Abstract
Abstract An improved diazonium coupling procedure is described for measurement of L-aspartate: 2-oxoglutarate aminotransferase (EC 2.6.1.1, formerly termed glutamic oxalacetic transaminase) activity in serum. A relatively pH-stable dye, 5-chloro-2-methoxyaniline-1-diazonium chloride (Fast Red RC) is used, with dimethylformamide and methanol to enhance and stabilize color development. A 15-min incubation is part of the procedure, and color development stabilizes within 5 min.
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