Abstract
With the increase in population and the advancement of medicine, people even more hope that their offspring will be healthier. DNA fragmentation rate is currently one of the more common indicators for evaluating sperm fertility and predicting the outcome of pregnancy. In order to evaluate the technical performance and application value of commercial sperm DFI (DNA fragmentation index) quality control products in the flow cytometry sperm chromatin structure analysis (SCSA), this paper uses flow cytometry to test commercial sperm DFI quality control products (Celula) and laboratory routine self-made DFI quality control products. In this paper, the quality control of new commercial sperm DFI and laboratory-made quality control were compared and tested for 30 consecutive days. And this paper monitors the response of commercial quality control products to the interference of key reagent parameters. This paper compares the stability of the test results of two quality control products and their sensitivity to the interference of key performance parameters of the detection reagent. Experimental results show that commercial sperm DFI quality control products can simulate sperm DNA damage to achieve accurate detection of DNA integrity. The stability of commercial sperm DFI quality control (CV = 2.47%) is better than that of laboratory-made quality control (CV = 11.22%). The new commercial sperm DFI quality control product can sensitively detect changes in the concentration of acidified solution and staining solution at the same time. It can effectively control the quality of detection reagents and experimental procedures. The new commercial sperm DFI quality control product can effectively control sperm DNA integrity testing. It can be used as an external quality control product for quality control of test results to ensure that more accurate test results are provided to the clinic.
Highlights
IntroductionSperm DNA fragmentation has been extensively studied for more than a decade
In the 1980s, the concept of sperm DNA fragmentation related to infertility was introduced, and the first DNA fragmentation test, sperm chromatin structure analysis (SCSA), was performed. e TUNEL test was introduced in the 1990s, followed by other test methods. e link between DNA fragmentation in sperm and miscarriage has been extensively studied, which has stimulated the demand for treatment tools for these patients. is has caused increased interest in the etiology of DNA damage
From the results of stream clustering, it can be found that P1 and P2 can be effectively separated, and the calculation process of sperm DNA fragmentation index (DFI) can be simulated in the same way. erefore, the commercial sperm DFI quality control product can effectively simulate the DNA damage classification and the calculation of the fragment index for the DNA integrity test of the sperm sample
Summary
Sperm DNA fragmentation has been extensively studied for more than a decade. In the 1940s, people discovered the uniqueness of the sperm-protein complex that stabilizes DNA. In the 1950s and 1960s, people studied the relationship between unstable chromatin structure and infertility. In the 1970s, the effect of inducing DNA damage was studied. In the 1980s, the concept of sperm DNA fragmentation related to infertility was introduced, and the first DNA fragmentation test, sperm chromatin structure analysis (SCSA), was performed. E TUNEL test was introduced in the 1990s, followed by other test methods. E link between DNA fragmentation in sperm and miscarriage has been extensively studied, which has stimulated the demand for treatment tools for these patients. Is has caused increased interest in the etiology of DNA damage. The lack of necessary quality control products is one of the reasons why laboratory quality control cannot be carried out
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