Technical and economical feasibility of a rotifer recirculation system

Abstract

A feasibility study was performed on the use of a recirculation system for the mass culturing of rotifers at industrial level. Rotifer culture systems with a culture volume of 750 l were operated at three different stocking densities (3000, 5000 and 7000 individuals ml −1) in a completely closed recirculation system. At all operating rotifer densities, a reliable production of 2.2 billion rotifers could be obtained on a daily basis during 3 weeks. Excellent water quality was maintained by the use of protein skimmers, the use of ozone and a submerged biofilter. The microbial counts remained stable during the whole culture period (10 6 CFU ml −1 on marine agar and 10 4 CFU ml −1 on TCBS after 15 and 23 days, respectively). No difference in HUFA and protein content were obtained between rotifers harvested from the recirculation system or from a conventional batch culture system. Compared to a commercial batch culture system, the use of a recirculation system can contribute to a 43% saving on the capital investment and the annual operation cost. By using this system, capital investment cost is considerably reduced by 46%. Savings are also made on labour cost (65%) and feed cost (21%) during a 1-year production. In general terms, it can be stated that by using a simple recirculation system, a cost-effective technology and a reliable rotifer culture can be obtained.