Abstract
BackgroundTea (Camellia sinensis) infusions are widely consumed beverages with numerous health benefits. However, physiological and molecular responses mediating these activities are poorly understood.MethodThree replicates of 4TI cancer cell suspension (2.0 × 105 cells/ml) were challenged in vitro with various concentrations of green, black and purple tea infusions to asseses their cytoxicity and associated differentially expressed genes in the cells. Inhibitory activity was tested by using serial dilutions of respective tea infusions in a 96 well ELISA plate.ResultsGreen tea had the highest inhibition on 4TI cells proliferation at a concentration of IC50 = 13.12 μg/ml. Further analysis of the 4TI cancer cell line treated with tea using 454 pyrosequencing generated 425,696 reads with an input mean length of 286.54. Trimmed sequences were imported on a CLC genomic workbench v7.03 and annotated on a reference mouse genome (Mus musculus strain C57BL/6 J). Results revealed a differential expression of apoptosis related genes in the transcriptome. Casp8, Casp9, Casp3, Casp6, Casp8AP2, Aifm1, Aifm2 and Apopt1 genes were significantly upregulated indicating the process of apoptosis was initiated and executed.ConclusionThese findings on caspases offer valuable information on the mechanism of tea as an anticancer agent and will contribute to further research in future novel treatments.
Highlights
Tea (Camellia sinensis) infusions are widely consumed beverages with numerous health benefits
Results from this study revealed that tea had an effect on cell growth by disrupting the monolayer formation which was evidenced by a reduction in the cell to cell contact and this was the reason why there was an ultimate reduction in the spreading of the cells (Figs. 3, 4 and 5)
Mapping of the sequences obtained from the 454 pyrosequencing platform onto the reference mouse genome Mus musculus strain C57BL/6 J and subsequent differential gene expression analyses revealed that green, black and purple tea extracts had specific responsive genes in the 4TI cancer cells
Summary
Tea (Camellia sinensis) infusions are widely consumed beverages with numerous health benefits. Method: Three replicates of 4TI cancer cell suspension (2.0 × 105 cells/ml) were challenged in vitro with various concentrations of green, black and purple tea infusions to asseses their cytoxicity and associated differentially expressed genes in the cells. Functional foods and naturally occurring polyphenols have physiological and pharmacological activities including their well characterized antioxidant properties [1,2,3]. Tea is rich in polyphenols associated with numerous pharmacological properties such as; antimicrobial [5], anti-inflammatory [6], anti-aging [7, 8] antimalarial [9] and antioxidant activity [1, 3, 10].
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