Abstract
An analytical TD-GC-MS method was developed and used for the assessment of volatile organic compounds (VOCs) released from the blood plasma of dogs with/without cancer. VOCs released from 40 samples of diseased blood and 10 control samples were compared in order to examine the difference between both sample groups that were showing qualitatively similar results independent from the disease’s presence. However, mild disturbances in the spectra of dogs with cancer in comparison with the control group were observed, and six peaks (tentatively identified by comparison with mass spectral library as hexanal, octanal, toluene, 2-butanone, 1-octen-3-ol and pyrrole) revealed statistically significant differences between both sample groups, thereby suggesting that these compounds are potential biomarkers that can be used for cancer diagnosis based on the blood plasma TD-GC-MS analysis. Statistical comparison with the application of principal component analysis (PCA) provided accurate discrimination between the cancer and control groups, thus demonstrating stronger biochemical perturbations in blood plasma when cancer is present.
Highlights
A large number of biochemical reactions proceed with great accuracy inside the body of live organisms
Blank chromatograms are obtained by TD-gas-chromatography mass spectrometry (GC-MS) analysis of the Tenax GR adsorption tube, after blowing He gas at the same flow rate of 50 mL/min for 10 min without sample
Taking into account reference studies and the established fact that hundreds of different chemical compounds are present in blood or blood plasma, our current result reveals much less, but this amount is reasonable if one considers the small amount of sample used for volatile organic compounds (VOCs) collection
Summary
A large number of biochemical reactions proceed with great accuracy inside the body of live organisms. Fundamental researches involve experiments to establish which volatile organic compound (VOC) can be released or consumed by cancer cell cultures [15,16,17], or, alternatively, which biomarkers are released as a response to VOC exposure [18] These studies are inspired by the successful results obtained in similar tests with other organisms such as algae [19] and bacteria [20], and their identification based on the exhaled VOC patterns. To the best of our knowledge, this is the first study representing TD-GC-MS analysis of dog blood plasma VOCs as potential biomarkers of cancer independent of the disease
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